Figure 2.

RAG-1 expression of total unseparated thymocytes and CD1a⁻ and CD1a⁺ CD8 (A) and CD4 SP (B) postnatal thymocytes. Thymocytes were depleted with magnetic beads for >97% of CD4 or CD8 positive cells. CD4⁻ cells were labeled with CD8 FITC, CD1a PE, and CD3 TRC and sorted from CD1a⁺ and CD1a⁻ CD3⁺CD8⁺ SP cells (A). Likewise, CD1a⁺ and CD1a⁻ CD4⁺ SP cells were sorted from CD8⁻ cells stained with CD4 FITC, CD1a PE, and CD3 TRC (B).