Table 1.
Thymocytes | Number of positive wells | |||||
---|---|---|---|---|---|---|
1 cell/well | 5 cells/well | 25 cells/well | ||||
Exp. 1 | ||||||
CD1+DP | 0/144 | 0/24 | 0/12 | |||
CD1−DP | 34/144 (24%) | 15/24 (63%) | 12/12 (100%) | |||
CD1+CD4SP | 1/144 (1%) | 0/24 | 0/12 | |||
CD1−CD4SP | 59/144 (41%) | 23/24 (96%) | 12/12 (100%) | |||
CD1+CD8SP | 0/144 | 0/24 | 1/12 (8%) | |||
CD1−CD8SP | 49/144 (34%) | 24/24 (100%) | 12/12 (100%) | |||
Exp. 2 | ||||||
CD1+CD4SP | 0/60 | 0/24 | NT | |||
CD1−CD4SP | 15/60 (25%) | 22/24 (92%) | 6/6 (100%) | |||
CD1+CD8SP | 0/60 | 0/24 | 0/6 | |||
CD1−CD8SP | 16/60 (27%) | 20/24 (83%) | 6/6 (100%) |