Table 2.
Expression of CD27, CD69, and Bcl-2 on CD1a+ versus CD1a− CD4 and CD8 SP Postnatal Thymocytes
| Percentage of thymocytes expressing | ||||||||
|---|---|---|---|---|---|---|---|---|
| CD27 | CD69 | Bcl-2 | ||||||
| % | % | % | ||||||
| CD3CD4SP | CD1+ | 62 | 95 | 99 | ||||
| CD1− | 100 | 67 | 100 | |||||
| CD3CD8SP | CD1+ | 88 | 100 | 96 | ||||
| CD1− | 100 | 72 | 100 | |||||
Thymocytes were stained with CD4TRC/CD8PE or CD8TRC/CD4 PE and FACStar® sorted for TRC+PE− CD4 and CD8 SP cells. Sorted cells were stained with CD27 FITC, CD69 FITC, Bcl-2 FITC, and CD3 FITC against CD1a PE or with control antibodies. The percentages of CD1 positive and negative cells CD4 and CD8 SP thymocytes that express the above markers were determined by FACScan® analysis. The indicated percentages are those on CD3highSP cells.