Figure 2.

Both wild-type Jak3 transgenes reconstitute T cell, but not B cell, development in Jak3 ^−/− mice. (A) The bone marrow, thymus, and spleen cells of Jak3 ^+/−, Jak3 ^−/−, Jak3 ^−/− (tg^kd), and 35-d-old Jak3 ^−/− (tg^thy+spl) and Jak3 ^−/− (tg^thy) mice were stained with the indicated antibodies and analyzed by flow cytometry. Staining is shown on a logarithmic scale of fluorescence intensity. Numbers in the quadrants indicate subpopulation percentages. The dot plots are representative of average staining profiles, although some Jak3 ^−/− individuals had greatly increased CD4⁺/CD8⁺ ratios in the thymus and spleen. (B) The total cellularity of bone marrow, thymus, and spleen of mice analyzed in these experiments is indicated. For each organ, Jak3 ^+/−, lane 1; Jak3 ^−/−, lane 2; Jak3 ^−/− (tg^thy+spl), lane 3; Jak3 ^−/− (tg^thy), lane 4; and Jak3 ^−/− (tg^kd), lane 5 are shown. Note the reconstitution of normal thymocyte cellularity by both wild-type, but not the kinase-dead, Jak3 transgenes. Data shown are representative of greater than six independent experiments.