Figure 4.

Functional responses of Jak3 ^−/− (tg^thy) T cells are restored in the thymus, but lost in the periphery. (A) Thymocytes and splenocytes from Jak3 ^+/−, Jak3 ^−/−, Jak3 ^−/− (tg^thy+spl), and Jak3 ^−/− (tg^thy) mice were analyzed for proliferation (top) and IL-2 (middle) or IL-3 (bottom) secretion in response to stimulation with anti-CD3 plus anti-CD28 antibodies. All data shown are from one 35-d-old Jak3 ^−/− (tg^thy+spl) mouse and one 35-d-old Jak3 ^−/− (tg^thy) mouse (designated B). IL-2 secretion data from splenocytes of two additional Jak3 ^−/− (tg^thy) mice, one 25 d of age (designated A) and one 42 d of age (designated C), are also shown. All values are mean ± SD. Overall, no statistically significant differences between Jak3 ^+/−, Jak3 ^−/− (tg^thy+spl), and Jak3 ^−/− (tg^thy) thymocytes were observed for IL-2 or IL-3 secretion responses. (B) Thymocytes and splenocytes from Jak3 ^+/−, Jak3 ^−/−, and Jak3 ^−/− (tg^kd) mice were stimulated with antibodies to CD3 plus CD28. Proliferative, IL-2 secretion, and IL-3 secretion responses are shown. All values are mean ± SD. For proliferation assays, all cell populations cultured in media alone gave responses of <500 cpm. For cytokine assays, cells cultured in media alone secreted undetectable levels of IL-2 (<0.02 U/ml) and IL-3 (<0.01 U/ml). Data are representative of 2–9 independent experiments.