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IL-12 p75 production by D1 cells pretreated or not with TNFα upon presentation of OVA protein to the specific hybridoma BO97.10 (striped bars or in the absence of the hybridoma open bars). IL-12 p75 was measured using an ELISA as described in Material and Methods. Il-12 p75 was quantified from two to three titration points using standard curves generated by purified recombinant mouse IL-12 and results expressed as cytokine concentration in pg/ml. Detection limit was 10 pg/ml.
