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. 1997 Jan 20;185(2):263–272. doi: 10.1084/jem.185.2.263

Figure 4.

Figure 4

Effect of Fas (CD95) on negative selection of thymocyte subpopulations. (A) Purified cells from B6 or B6 lpr/lpr mice were cultured for 20 h on plates coated with either anti-TCR mAb alone (20 μg/ml) or with anti-TCR (10 μg/ml) plus anti-CD28 (20 μg/ml) mAb. Where indicated, Fas–Ig fusion protein (provided by Dr. D. Lynch; 10 μg/ml) was added to the cultures. (B) Relative sensitivity of normal B6 and B6 lpr/lpr CD4+8+ and HSAhi CD4+8− cells to TCR–CD28-mediated apoptosis in vitro. Cells were cultured for 20 h on plates coated with various concentrations of anti-TCR mAb ± a fixed concentration of anti-CD28 mAb as described in Fig. 3. Apoptosis was measured by TUNEL staining. The data represent the mean of two separate experiments.