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. 1997 Jan 20;185(2):219–230. doi: 10.1084/jem.185.2.219

Figure 6.

Figure 6

Anergy induction by stimulation with wild-type ligand in the presence of anti-CD4 antibody. (a) A.E7 T cells were incubated with I-Ek-expressing L cells without or with PCC(81–104) (100 nM) and antiCD4 mAb (1:200 dilution of supernatant) for 24 h. T cells were then isolated and rested for 7 d. At this point, T cells were restimulated with I-Ek-transfected L cells and PCC(81–104) (1 μM) for 24 h and IL-2 production was measured by ELISA. (b) Effect of variation in the concentration of anti-CD4 mAb in the primary culture on the extent of anergy induction. A. E7 cells were treated as in (a), but using various concentrations of anti-CD4 antibody (1:200, open squares; 1:2,000, closed diamonds; 1:20,000, half-filled squares; 1:200,000, half-filled diamonds; no antiCD4, closed triangles). After rest, each of these cell populations was restimulated as in (a) and IL-2 in the medium measured after 24 h by ELISA.