Table 1.

Anticonidial and Phagocytic Potential of Wild-type and X-CGD Alveolar Macrophages

Experimental group		Phagocytic index		Percent phagocytosis		Percent of conidia killed
Wild type		2.2 ± 0.3		61 ± 2		86 ± 18
X-CGD		2.1 ± 0.4		55 ±13		92 ± 10

Alveolar macrophages were allowed to ingest conidia for 45 min at 37°C (1:5 macrophage to conidia ratio), and then incubated an additional 6 h at 37°C. Percent killing of ingested conidia was then determined by lysing macrophages and plating in at least triplicate cultures on Sabaroud agar. Phagocytic index (the ratio of ingested conidia to macrophages) and percent phagocytosis (the percentage of macrophages ingesting at least one conidia) were determined by counting at least 200 cells in Wright-Giemsa stained cytospins. Data are expressed as mean ± SD of two independent experiments using alveolar macrophages pooled from three to five mice per experiment.