Table 1.
Somatic Hypermutation in BP2, CPM1, and CPM2 Hybridomas
| Name of hybridomas* | Isotype | Mutations in VH | Mutations in Vκ | |||||
|---|---|---|---|---|---|---|---|---|
| BP2 hybridomas expressing hybrid H chain loci | ||||||||
| Primary | ||||||||
| 4ABP2-8 | G7 | IgG2b | 0 | 1 | ||||
| D10 | IgG2b | 0 | 0 | |||||
| E4 | IgG2b | 0 | 1 | |||||
| 4ABP2-16 | G6 | IgG1 | 1 | 3 | ||||
| A5 | IgG1 | 2 | 0 | |||||
| AABP2-2 | E3 | IgG2b | 2 | 3 | ||||
| 3APB2-7 | H3 | IgG2b | 0 | 2 | ||||
| Secondary and tertiary | ||||||||
| AABP2-28a | E5 | IgG1 | 1 | 1 | ||||
| 3ABP2-59 | A12 | IgG1 | 7 | 1 | ||||
| D7 | IgG2b | 2 | 1 | |||||
| 4ABP2-13 | H7 | IgG1 | 4 | 0 | ||||
| 3ABP2-44 | H8 | IgG1 | 1 | 2 | ||||
| BP2 hybridomas expressing endogenous H chain loci | ||||||||
| Primary | ||||||||
| 4ABP2-8 | E7 | IgM | 3 | 0 | ||||
| AABP2-10 | H7 | IgG1 | 5 | 1 | ||||
| 3ABP2-7 | B1 | IgG2b | 4 | 4 | ||||
| 4ABP2-23 | F10 | IgM | 1 | 3 | ||||
| D2 | IgG1 | 3 | 1 | |||||
| Secondary and tertiary | ||||||||
| 3ABP2-43 | D3 | IgG1 | 8 | 1 | ||||
| A12 | IgG1 | 11 | 2 | |||||
| 3ABP2-45 | G2 | IgG1 | 3 | 2 | ||||
| CPM hybridomas expressing hybrid H chain loci | ||||||||
| Primary | ||||||||
| 3ACPM2-23 | B2 | IgG1 | 3 | 1 | ||||
| G6 | IgG1 | 5 | 3 | |||||
| 4ACPM1-2 | D9 | IgG1 | 2 | 4 | ||||
| G3 | IgG1 | 0 | 4 | |||||
| C6 | IgG3 | 0 | 1 | |||||
| 4ACPM1-3 | D5 | IgG2b | 3 | 2 | ||||
| F1 | IgG3 | 12 | 2 | |||||
*
The first four–five characters before the dash in each hybridoma name indicate the name of the mouse from which that hybridoma was derived. Among the BP2 hybridomas, the groups (4ABP2-8 D10, E4, E7, G7); and (3ABP2-7 B1, H3) were derived from single mice. The groups (3APB2-44 H8, 3ABP2-45 G2); (AABP2-2 E3, AABP2-10 H7) and (4ABP2-16 G6, A5, 4ABP2-23 D3, A12) were derived from littermates immunized with the same dose and preparation of antigen and sacrificed for fusion on the same day.