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. 1997 Jan 20;185(2):305–316. doi: 10.1084/jem.185.2.305

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Neuronal cell culture and lineage specific single cell RT-PCR. Neurons from embryonic hippocampi differentiated on top of a monolayer of astrocytes (A). Double immunofluorescence labeling for cell lineage-specific cytoskeletal markers MAP2 for neurons (B) and GFAP for astrocytes (C). Gene transcripts for MAP2 and GFAP amplified by single-cell patch–clamp RT-PCR from individual neurons (lanes 1–3) and astrocytes (lanes 4–6) on ethidium bromide stained agarose-gel (D). Lane N and lane M show negative controls of PCR-amplification and molecular weight marker X174/Hae III, respectively. Bar, (A–C) 20 μm.