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. 1997 Jan 20;185(2):305–316. doi: 10.1084/jem.185.2.305

Figure 7.

Figure 7

Restriction site analysis of amplified PCR-fragments and MHC class I heavy chain induction by TNF-α. (A) PCR-fragments amplified from IFN-γ plus TTX treated individual neurons, digested by restriction enzymes selected from the published sequence. PCR-fragments for GAPDH (428 bp) cut into 324 bp and 104 bp, for β2-microglobulin (332 bp) into 206 bp and 126 bp, for MHC class I heavy chain (198 bp) into 160 bp and 38 bp. PCR-fragments for TAP1 (300 bp) cut into 194 bp and 106 bp. PCR-fragments for TAP2 (264 bp) cut into 192 bp and 72 bp. All analyzed PCR-fragments were in agreement with the published sequences. (B) Gene transcripts for GAPDH, β2-microglobulin and MHC class I heavy chain coamplified from individual neurons (lanes 1 to 10) treated for 72 h with TNF-α. Lane N and lane M show negative control of PCR-amplification and molecular weight marker ΦX174/Hae III, respectively.