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. Author manuscript; available in PMC: 2009 Jan 5.
Published in final edited form as: Virology. 2007 Sep 29;370(1):184–193. doi: 10.1016/j.virol.2007.08.011

Figure 2. Viral Replication in T cells.

Figure 2

(A) Replication of SIV variants in pig-tailed macaque dendritic cell-T cell co-cultures. Monocyte-derived dendritic cells were co-cultured with autologous T cells in triplicate and infected with chimeric and parental viruses as described (Kimata et al., 2004). Viral replication was monitored by measuring SIV p27 levels in supernatants of the co-cultures by ELISA. (B) Replication of SIV variants in pig-tailed macaque CD4+ T cells costimulated with anti-CD3/anti-CD28 antibodies. T cells were infected with virus in triplicate, washed, and incubated in plates coated with anti-CD3 and anti-CD28 for 24 hours. Cultures were allowed to continue for 2 weeks. Supernatants were harvested and media replaced every 3-4 days. In both A and B, average p27 levels ± SD are shown. Data are representative of six and two independent experiments in A and B, respectively.