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. 1997 Feb 17;185(4):583–600. doi: 10.1084/jem.185.4.583

Figure 5.

Figure 5

CD4+ T cells from MBP-specific TCR transgenic mice express less membrane TNF-α and LT-αβ than do those from HA-specific TCR transgenic mice. Expression of CD69, Fas, FasL, mTNF, and LT-αβ on splenocytes from either the MBP-specific TCR transgenic line cultured with 50 μM Ac1-11 (Ac1-11) or the hemagglutinin-specific TCR transgenic line (HA) cultured in vitro with 0.2 μg/ml HA peptide 126-138 for 48 h. Live cells from the 48-h cultures were collected on a Ficoll gradient and washed two times. Cells were then stained with a biotinylated antibody to CD69, a phycoerythrin-conjugated antibody to Fas, or Fas/Fc, TNFR/Fc, or LT-βR/Fc fusion proteins as described in Materials and Methods. Histograms display only large, blasting cells as determined by forward scatter. For CD69 and Fas, dashed lines in plots indicating levels of CD69 or Fas, respectively, of naive cells from PBS-injected MBP-specific TCR transgenic mice. For FasL, mTNF, and LT-αβ expression, dashed lines indicate fluorescence intensity of cells incubated with only the biotinylated secondary antibody and streptavidin-conjugated Texas red as a negative control.