Figure 8.
The effect of the addition of anti–class II antibody (10-3.6) on in vitro differentiation of naive TCR transgenic mice T cells by 50, 5, and 0.5 μM Ac1-11[4Y]. Black bars represent responses of T cells cultured with 12.5 μg/ml 10-3.6 and Ac1-11[4Y], shaded bars those of T cells cultured with 3.125 μg/ml 10-3.6 and Ac111[4Y], and cross-hatched bars those of T cells cultured with Ac1-11[4Y] alone. Sorted naive T cells were cultured in 24-well plates with irradiated splenocytes as APCs with various amounts of 10-3.6 and Ac1-11[4Y]. IL-2 production in the primary stimulation was determined on culture supernatant 48 h after initial stimulation with Ac1-11[4Y] and 10-3.6. After a total of 7 d, viable cells were then washed and cultured with 10 μM Ac1-11[4Y] or medium only in 96-well round-bottom plates at 5 × 104 cells/well and 5 × 105 APCs/well for 48 h. IFN-γ and IL-4 production in the secondary stimulation was determined as described. (A) IL-2 production by naive T cells in the primary stimulation is decreased by addition of 10-3.6. (B) IFN-γ production by T cells in the secondary stimulation does not change significantly by addition of 10-3.6. (C) IL-4 production by T cells in the secondary stimulation increases 2–20-fold, depending on the initial concentration of Ac1-11[4Y].