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. 1997 Feb 17;185(4):731–744. doi: 10.1084/jem.185.4.731

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Expression of Egr-1 mRNA in double positive thymocytes. (A) Total thymocytes and CD4⁺8⁺ thymocytes (isolated by cell sorting, 95% DP) derived from the same animal, were assayed for expression of Egr and CD4 mRNA by competitive RT-PCR. Shown is the relative level of Egr-1 cDNA in the sample, normalized to the level of CD4 cDNA. (B) Total thymocytes derived from an MHC-deficient mouse were cultured with hamster immunoglobulin-coated or anti-CD3ε mAbcoated beads for 90 min before determination of Egr-1 and CD4 gene expression as in Fig. 1 C.