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. 1997 Feb 17;185(4):785–790. doi: 10.1084/jem.185.4.785

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Generation of eotaxin-disrupted mice. Shown in (A) is the eotaxin genomic locus, the targeting vector, and the targeted null locus. Vertical rectangles represent exons; black regions in the exons are deleted by the targeting strategy. The targeting vector contains a neomycin-resistant gene (neo) and a herpes simplex virus thymidine kinase (HSV–TK) gene in tandem. In (B), Southern analysis of BamHI-digested genomic DNA from the offspring of a heterozygous cross. Wt and null indicate wild-type and targeted locus, respectively. (C) shows a Northern analysis of skin total RNA (20 mcg) from homozygote null (−/−), heterozygote (+/−), and wild-type mice (+/+). The Northern blot was hybridized with a full-length murine eotaxin cDNA or 28S rRNA probe. Restriction enzyme abbreviations: N, NotI; B, BamHI; Xh, XhoI; R5, EcoRV; X, XbaI.