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. 1997 Feb 17;185(4):653–662. doi: 10.1084/jem.185.4.653

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5′_λ5 is active in BM but not in spleen, thymus, heart, muscle, or liver. 5′_λ5 hu-CD25: 5′_λ5 inserted upstream of the cDNA encoding human IL-2–R (hu-CD25; reference 21) followed by exon 2, intron 2, exon 3 (solid boxes), and polyA site (hatched box) from human β-globin (11). The 5′_λ5 hu-CD25 was used to establish transgenic mice. (A) BM, spleen, and thymus cells were analyzed by FACS^® using antibodies detecting mouse CD19 or CD4 and hu-CD25. Data from nontransgenic (tg−) and transgenic (tg+) littermates from founder 82 are shown. (B) Total RNA from heart, muscle, liver, spleen, and BM was isolated and analyzed for expression of hu-CD25 and HPRT by RT-PCR analysis. Arrows denote the 185 and 220-bp fragments of hu-CD25 or HPRT, respectively.

5′_λ5 is active in BM but not in spleen, thymus, heart, muscle, or liver. 5′_λ5 hu-CD25: 5′_λ5 inserted upstream of the cDNA encoding human IL-2–R (hu-CD25; reference 21) followed by exon 2, intron 2, exon 3 (solid boxes), and polyA site (hatched box) from human β-globin (11). The 5′_λ5 hu-CD25 was used to establish transgenic mice. (A) BM, spleen, and thymus cells were analyzed by FACS^® using antibodies detecting mouse CD19 or CD4 and hu-CD25. Data from nontransgenic (tg−) and transgenic (tg+) littermates from founder 82 are shown. (B) Total RNA from heart, muscle, liver, spleen, and BM was isolated and analyzed for expression of hu-CD25 and HPRT by RT-PCR analysis. Arrows denote the 185 and 220-bp fragments of hu-CD25 or HPRT, respectively.