Table 1.

Occurrence of AIHA in Anti-RBC AutoAb Tg Mice in Different Breeding Environments

		ConV		SPF		GF		ConV* (LPS injected)		GF/ConV‡		SPF/ConV‡		GF* (LPS injected)
Frequency of autoimmune
hemolytic anemia %		46		0		0		100		33		33		25
Number of mice with
autoimmune hemolytic anemia		29		0		0		13		2		4		2
Number of tested mice		63		26		30		13		6		12		8

We considered Tg mice with hematocrit values below 40% as AIHA mice and examined the occurrence of AIHA in 6- to 8-wk-old anti-RBC autoAb Tg mice in different breeding conditions: GF, SPF, and conventional conditions (ConV).  

^* Anti-RBC autoAb Tg mice bred in the conventional or GF condition were examined 7 d after oral administration of 100 μg of LPS.  

^‡ GF Tg mice or SPF Tg mice were transferred to the conventional condition, bred there for 4–6 wk, and then examined.