Table 2.

Soluble Factors Released by LPS-treated MDM Inhibit the Replication of NSI HIV-1 Strains in T Lymphocytes

Culture		Supernatant added		Polymixin		HIV-1 p24 Ag release
						Ba-L		181		5233
								pg/ml
T cells+HIV-1		−		−		2,155		8,755		7,057
T cells+HIV-1		−		+		2,355		6,390		7,592
T cells+HIV-1+LPS		−		−		2,344		7,795		6,793
T cells+HIV-1+LPS		−		+		2,086		7,885		7,738
T cells+HIV-1		Untreated MØ		+		2,225		8,927		7,462
T cells+HIV-1		LPS-treated MØ		+		47		281		7,198

Lymphocytes from healthy donors were infected in vitro with two NSI HIV-1 strains, HIV-1_Ba-L and HIV-1₁₈₁, or with an SI strain, HIV-1₅₂₃₃, in the presence or absence of LPS (1 μg/m), LPS-conditioned supernatants (100% vol/vol) or polymixin B, sulfate (15 μg/ml). Supernatants from infected cultures were harvested 5 d after infection, and assayed by ELISA for p24 Ag secretion.