Table 4.
Antibody-mediated Depletion of C–C Chemokines Neutralizes the HIV Suppressive Activity of LPS-conditioned Supernatants
| Culture | Supernatant added | Polymixin | Depletion | HIV-1 p24 Ag | ||||
|---|---|---|---|---|---|---|---|---|
| pg/ml | ||||||||
| MDM+HIV-1 | − | − | − | 4,516 | ||||
| MDM+HIV-1 | − | + | − | 4,426 | ||||
| MDM+HIV-1+LPS | − | − | − | 597 | ||||
| MDM+HIV-1+LPS | − | + | − | 4,500 | ||||
| MDM+HIV-1 | Untreated MØ | + | − | 5,739 | ||||
| MDM+HIV-1 | LPS-treated MØ | + | − | 176 | ||||
| MDM+HIV-1 | LPS-treated MØ | + | Anti-chemokines | 3,597 | ||||
| MDM+HIV-1 | LPS-treated MØ | + | Normal goat IgG | 806 |
MDM from healthy donors were infected in vitro with HIV-1Ba-L, in the presence of LPS-conditioned supernatants (100% vol/vol), undepleted or depleted of monokines by adsorption on specific neutralizing antibodies or control IgG immobilized on plastic. Polymixin B sulfate was added at a concentration of 15 μg/ml. Supernatants from infected cultures were harvested after 4 d of culture, and assayed by ELISA for p24 Ag secretion. The table shows the results of a representative experiment.