Figure 3.

Inhibition by LPS of MCP-1 binding and chemotaxis. (A) Binding. fresh human monocytes were incubated for 1–5 h in the presence or absence of 100 ng/ml of LPS before [125I]MCP-1 binding assay. Results are expressed as percent of bound on total counts. (B) Chemotaxis. Fresh human monocytes were stimulated with 100 ng/ml of LPS and chemotactic response to MCP-1 (100 ng/ml) was measured after different periods as indicated.