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. 1997 Mar 3;185(5):825–832. doi: 10.1084/jem.185.5.825

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Cytokine profiles of cord blood derived Th1 and Th2 lines and of established human T cell clones. (A) IFN-γ and IL-4 production by Th1 and Th2 lines generated from cord blood lymphocytes. Cells were washed on day 10 after priming, 10⁵ cells were restimulated in 96well round-bottom plates for 24 h with plate-bound anti-CD3 antibodies (clone TR66; reference 19), to determine IFN-γ and IL-4 in culture supernatants by ELISA assays. The results shown are representative of five independent experiments. (B) IFN-γ and IL-4 production by established Th1 (CB13) and Th2 (CB21) clones.