Skip to main content
. 1997 Mar 3;185(5):817–824. doi: 10.1084/jem.185.5.817

Figure 1.

Figure 1

Figure 1

IL-12R β2 subunit mRNA is detected in Th1 but not Th2 cells. (A) Naive CD4+ T cells were purified by FACS® from unimmunized DO11.10 TCR-transgenic mice as described in Materials and Methods (5), activated with OVA peptide and APCs under either Th1- or Th2-inducing conditions and allowed to develop for 7 days. On day 7, the Th1 and Th2 cells were washed, restimulated and allowed to proliferate for 7 or 9 d when cells were harvested and total cellular RNA was isolated. As tissue controls, total cellular RNA was isolated from the B cell hybridoma TA3 and the fibroblast cell line L929. Northern blot analysis was performed using as probes the full-length murine IL-12R β2 subunit cDNA (top), the full-length murine IL-12R β1 subunit cDNA (middle), and the GAPDH cDNA (bottom). (B and C). Total cellular RNA was isolated from naive T cells after purification by FACS®. Naive T cells isolated by cell sorting were activated to induce Th1 or Th2 development, and harvested on days 3, 5, and 7 after primary antigen activation. Total cellular RNA was examined by Northern analysis as described above for IL-12R β2 subunit cDNA (top), the IL-12R β1 subunit cDNA (middle), or pHE7 cDNA (bottom).