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. 1997 Mar 3;185(5):909–920. doi: 10.1084/jem.185.5.909

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Lysis of targets expressing wt or ss⁻ env protein by the envspecific CD8⁺ CTL clone A42.46. Autologous B-LCL were infected with the indicated vaccinia expression vectors 2 h before assay (diamonds) or were infected for 2 h and then incubated overnight at 37°C before assay (squares). Infected cells were then used as targets for the env-specific CD8⁺ CTL clone A42.46 in a standard ⁵¹Cr release assay at an E/T ratio of 10:1. One set of targets was infected in the presence of 0.1 mM cycloheximide (CHX, circles). The experiment was repeated three times with similar results. Data from a representative experiment are shown.

Lysis of targets expressing wt or ss⁻ env protein by the envspecific CD8⁺ CTL clone A42.46. Autologous B-LCL were infected with the indicated vaccinia expression vectors 2 h before assay (diamonds) or were infected for 2 h and then incubated overnight at 37°C before assay (squares). Infected cells were then used as targets for the env-specific CD8⁺ CTL clone A42.46 in a standard ⁵¹Cr release assay at an E/T ratio of 10:1. One set of targets was infected in the presence of 0.1 mM cycloheximide (CHX, circles). The experiment was repeated three times with similar results. Data from a representative experiment are shown.