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. 1997 Mar 3;185(5):893–900. doi: 10.1084/jem.185.5.893

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Analysis of LN T cells from H-Y⁺/ζ Tg mice. Immunofluorescence and multicolor FCM analysis of LN cells from adult female and male H-Y⁺/ ζ-3 ITAM Tg or H-Y⁺/ζ-0 ITAM Tg mice. Also shown are LN cells from a nontransgenic C57BL/6 (H-Y⁻/ζ^+/+) mouse. Threecolor FCM was performed on total LN cells after staining with FITC– anti–H-Y clonotypic antibody (T3.70), anti-CD8–PE and anti-CD4– biotin, followed by streptavidin–red 670. CD4 versus CD8 two-color profiles are displayed on total (left) or gated T3.70⁺ (right) cells. Numbers in the regions reflect the percentage of total cells found in that region.