Figure 5.

Calcineurin regulation of Crz1p–Nmd5p binding requires resides on either side of the Crz1p NLS. 200 μg yeast cytosol from crz1Δ strains ASY472 (WT) and ASY475 (cnb1Δ) expressing pAMS478 (N-term), pRSP37 (C-term), and pAMS490 (SRRΔ) were incubated with 50 μl GST–Nmd5p bound to glutathione resin. Equal amounts of the bound fractions were analyzed on an 8.5% SDS-PAGE followed by Western blotting using an anti-GFP antibody. For each of the GFP–Crz1p fusion proteins, its electrophoretic mobility in WT and cnb1Δ cells is shown (Input).