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. 2001 Sep 3;154(5):1007–1018. doi: 10.1083/jcb.200103107

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Copyright © 2001, The Rockefeller University Press

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Figure 6. — Surface MHC I is internalized and gains access to PIP₂ vacuoles in cells expressing EFA6 at all times but is only loaded into cells expressing Arf6 Q67L early in transfection. Hela cells were transfected with PH-GFP and EFA6 or Arf6 Q67L. At the indicated time (20 or 44 h), they were allowed to internalize MHC I antibody for 30 min and then were fixed and processed for visualizing internalized antibody. (Inset) Enlargement of box showing MHC antibody internalized into PIP₂-positive macropinosomes. Bars, 10 μm.