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. 2001 Sep 3;154(5):925–936. doi: 10.1083/jcb.200102093

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Copyright © 2001, The Rockefeller University Press

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Figure 2. — MCC inhibition of the APC/C requires hBUBR1. Purified mitotic APC/C was incubated in the presence of buffer (lane 1), MCC (lane 2), MCC that was mock depleted with nonimmune IgG (lane 3) or immunodepleted with hBUBR1 antibodies (lane 4), and then assayed for ubiquitin ligase activity. (A) Ubiquitination assay. Mock- and hBUBR1-immunodepleted supernatants were probed for hBUBR1 (top panel). Asterisk denotes the contaminant iodinated protein. (B) Quantification of the ubiquitination assay on phosphorimager.