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. 2001 Sep 3;154(5):1081–1088. doi: 10.1083/jcb.200102034

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Copyright © 2001, The Rockefeller University Press

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Figure 3. — DOC-soluble and -insoluble FNΔIII₁. CHOα5 cells were incubated with 50 μg/ml pFN or FNΔIII₁ for the indicated times and lysed in buffered DOC. DOC-soluble (A) and -insoluble (B) fractions were separated in 5% polyacrylamide-SDS gels without reduction and transferred to nitrocellulose. FN was detected on immunoblots with monoclonal anti-FN antibody IC3 and chemiluminescence reagents. Dimeric pFN and FNΔIII₁ are present (arrowhead) as well as high molecular mass multimers at the top of the stacking (bracket) and at the interface of the stacking and separating gels (arrow).