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. 2001 Sep 3;154(5):961–972. doi: 10.1083/jcb.200102073

Figure 4.

Figure 4.

Bradykinin-induced Ca2 + release in calreticulin-deficient cells. Cells were loaded with the fluorescent Ca2+ indicator fura-2 and stimulated with 200 nM bradykinin. K41, wild-type mouse embryonic fibroblasts; K42, calreticulin-deficient cells; K42CRT, K42 cells transfected with calreticulin expression vector. (A) Represents typical traces showing bradykinin (BK) stimulation of cells in a Ca2+-free medium. Arrows indicate the time of addition of bradykinin. (B) Basal [Ca2+]c in wild-type (white bar), calreticulin- deficient (black bar), and calreticulin-deficient cells transfected with calreticulin expression vector (hatched bar). The absolute values of [Ca2+]c were: 100 ± 12 nM (K41), 148 ± 10 nM (crt− /− K42), and 95 ± 8 nM (crt− /− + CRT, K42CRT). (C) Ca2+ released by bradykinin was significantly different in cell lines investigated. K41, wild-type mouse embryonic fibroblasts; K42, calreticulin-deficient cells; K42CRT, K42 cells transfected with calreticulin expression vector. Data are mean ± SE (n = 3).