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. Author manuscript; available in PMC: 2008 Jan 14.
Published in final edited form as: J Am Chem Soc. 2006 Aug 2;128(30):9809–9812. doi: 10.1021/ja0615425

Figure 4.

Figure 4

Top: the increase of TMR fluorescence monitored at 580 nm as the quadruplex formed by I:II is opened by III in 10 mM Tris·HCl (pH 7.4) and 100 mM NaCl at 20 °C. Data from runs performed in the presence of ligand HC at concentrations 0 (○), 5 μM (●), and 50 μM (■) are shown, along with single-exponential fits (solid lines). In each series the initial fluorescence intensity has been subtracted from each data point. The rate constants of opening, kobs, were found to be 0.0086, 0.0053, and 0.0027 s−1 at 0, 5, and 50 μM HC, respectively. Bottom: the variation of observed rate constant with concentration of HC for the hybridization of I:II to III at 20 °C in 10 mM Tris·HCl (pH 7.4) and 100 mM NaCl. The data are shown as open circles and fits to eq 4 as a solid line.