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. Author manuscript; available in PMC: 2009 Jan 15.
Published in final edited form as: Exp Cell Res. 2007 Sep 29;314(2):398–412. doi: 10.1016/j.yexcr.2007.09.015

Fig. 2.

Fig. 2

HGF’s effect on the mRNA levels of GSH synthetic enzymes depends on the cell density. RNA (15 μg/lane) samples from H4IIE cells plated at low (part A, 2.5x104 cells/cm2) or high (part B, 6.2x104 cells/cm2) densities and treated with HGF (10 ng/ml) for 0 to 16 hours as described in Methods were analyzed by Northern blot analysis with different cDNA probes. The same membranes were rehybridized with a 32P-labeled β-actin cDNA probe. Representative Northern blots are shown. Numbers below each blot refer to densitometric changes expressed as % of 0 hour.