Table 1.

Antibody Titers and Airway Responsiveness in Wild-type and IL-10^−/− C57BL/6 Mice

Sensization		Wild type						IL-10−/−
		Control		i.n.		i.p. and i.n.		Control		i.n.		i.p. and i.n.
						μg/ml
Serum IgE titers
Median		0.40		22.90*		3.60*		1.10		12.60*		2.30*
Range		0.2–2.1		3.8–80.7		1.3–7.9		0.3–2.7		1.9–52.7		1.3–4.0
n		16		15		11		16		11		11
						mg/ml
Serum IgG1 titers
Median		0.08		0.97*		0.25*		0.11		1.05*		0.33*
Range		0.02–0.18		0.56–1.62		0.08–0.40		0.02–0.43		0.56–1.62		0.17–0.87
n		11		14		14		12		15		15
						μg ACh/g bodyweight
PC200
Mean		2.24		0.22*		0.31*		1.13		0.30*		0.47*
Range		0.89–4.17		0.04–0.41		0.11–0.56		0.51–2.40		0.13–0.59		0.14–1.02
n		6		12		11		6		12		12

Antibody titers and airway responsiveness in WT and IL-10^−/− C57BL/6 mice. Total serum antibody titers and airway responsiveness were examined 4–6 d after the final i.n. challenge of C57BL/6 mice primed i.n. or i.p. Airway responsiveness is expressed as PC200, the amount of ACh required to double baseline resistance. Antibody titers and PC200 values from unsensitized WT and IL-10^−/− mice were not statistically different. Baseline airway resistance was similar in sensitized and unsensitized mice (data not shown). For the statistical comparison between PC200 values from experimental groups and controls, data from unsensitized IL-10^−/− and WT mice were combined.  

^* Data are significantly different from unsensitized control mice (P <0.05).