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Effects of sSLAM on Ig synthesis by purified B cells. (A) negatively selected total B cells or (B) sorted sIgD+ B cells were cultured in the presence or absence of anti-CD40 mAbs (10 μg/ml) and sSLAM at concentrations indicated for 12 d. Ig levels in the culture supernatants were measured by isotype-specific ELISA. The data represent mean ± SEM of quadruplicate cultures in four experiments (A) or triplicate cultures in two experiments (B).
