Table 2.

Human CII Determinants Recognized by T Cells from B10.M and B10.M-DR1 Mice

T cell determinant		Peptide*		Peptide sequence‡		T cell stimulation§
		hCII (247–261)		GPLGPKGQTGEBGIA		−
B10.M determinant		hCII (250–264)		GPKGQTGEBGIAGFK		+
		hCII (253–267)		GQTGEBGIAGFKGEQ		−
B10.M-DR1		hCII (256–270)		GEBGIAGFKGEQGPK		+
Dominant determinant		hCII (259–273)		GIAGFKGEQGPKGEB		+
		hCII (262–276)		GFKGEQGPKGEBGPA		+
		hCII (265–279)		GEQGPKGEBGPAGPQ		−
		hCII (283–297)		GPAGEEGKRGARGEB		−
B10.M-DR1		hCII (286–300)		GEEGKRGARGEBGGV		+
Subdominant		hCII (289–303)		GKRGARGEBGGVGPI		+
determinant		hCII (292–306)		GARGEBGGVGPIGPB		+
		hCII (295–309)		GEBGGVGPIGPBGER		−

^* Mimotope synthetic peptides. Sequence numbers are based on assigning the first amino acid of the human α1(II) chain as residue number 1.  

^‡ Synthetic peptides are aligned according to sequence overlap used in the Mimotope analysis. Alignment allows for deduction of determinant core (underlined text) when more than one peptide is stimulatory for T cells. Amino acids are represented by single letter code with B denoting hydroxyproline.  

^§ Stimulation data summarized from T cell proliferation data in Fig. 6.