Table 4.

DR1-restricted T Cell Hybridomas Are Specific for the hCII(262–270) Core Determinant

T cell hybridoma		IL-2*
		Human CII Mimotope Peptides‡
		CII(250–264)		CII(253–267)		CII(256–270)		CII(259–273)		CII(262–276)		CII(265–279)
DR1hCII-2		–§		–		2,560		2,560		1,280		–
DR1hCII-3		–		–		2,560		2,560		2,560		–
DR1hCII-5		–		–		640		1,280		1,280		–
DR1hCII-14		–		–		1,280		2,560		1,280		–
DR1hCII-19		–		–		2,560		>2,560		640		–

^* Production of IL-2 was measured as an indicator of T cell hybridoma stimulation in an antigen presentation assay using DR1-expressing APCs, (cell line L57). After 24 h of culture, supernatants were collected and tested for the presence of IL-2–dependent cell line HT-2, as described in Materials and Methods. Data are expressed as units of IL-2 per milliliter.  

^‡ Peptides were derived from the same Mimotope peptide synthesis used in Fig. 6.  

^§ –, <20 U/ml of IL-2 produced.