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. 1997 Apr 7;185(7):1193–1202. doi: 10.1084/jem.185.7.1193

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PCR analysis of E_δ and E_δmCore minilocus rearrangement. (A) Genomic DNA from thymi of wild-type E_δ mice from lines A, B, and C, and from E_δmCore mice from lines U, Y1, Z, and Y2 were amplified by PCR using primers 5 and 6 (top), primers 1 and 3 (middle) and primers 2 and 3 (bottom). Southern blots were developed using radiolabeled C_δ, V_δ1 or V_δ2 cDNA probes. The mice analyzed were A-26 (3 wk old), B-29 (2 wk old), C-306 (6 wk old), U-19 (5 wk old), Y1-83 (2 wk old), Z-92 (3 wk old), and Y287 (2 wk old). (B) Genomic DNA from the thymi of mice from lines A, U, Z, and Y2 were amplified by PCR using primers 5 and 6 (top), primers 1 and 4 (middle) and primers 2 and 4 (bottom). The mice analyzed were A-866, U-339, Z-419 and Y2-280 (all 4 wk old).

PCR analysis of E_δ and E_δmCore minilocus rearrangement. (A) Genomic DNA from thymi of wild-type E_δ mice from lines A, B, and C, and from E_δmCore mice from lines U, Y1, Z, and Y2 were amplified by PCR using primers 5 and 6 (top), primers 1 and 3 (middle) and primers 2 and 3 (bottom). Southern blots were developed using radiolabeled C_δ, V_δ1 or V_δ2 cDNA probes. The mice analyzed were A-26 (3 wk old), B-29 (2 wk old), C-306 (6 wk old), U-19 (5 wk old), Y1-83 (2 wk old), Z-92 (3 wk old), and Y287 (2 wk old). (B) Genomic DNA from the thymi of mice from lines A, U, Z, and Y2 were amplified by PCR using primers 5 and 6 (top), primers 1 and 4 (middle) and primers 2 and 4 (bottom). The mice analyzed were A-866, U-339, Z-419 and Y2-280 (all 4 wk old).