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. 1997 Apr 7;185(7):1193–1202. doi: 10.1084/jem.185.7.1193

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PCR analysis of E_δ and δE3,4 minilocus rearrangement. Genomic DNA from thymi of wild-type E_δ mice from lines A, B, and C, and from δE3,4 mice from lines JA, JE, and JG were amplified by PCR using primers 5 and 6 (top), primers 1 and 3 (middle) and primers 2 and 3 (bottom). Southern blots were developed using radiolabeled C_δ, V_δ1, or V_δ2 cDNA probes. The mice analyzed were A-765 (4 wk old), B-31 (8 wk old), C-114 (4 wk old), JA-81 (4 wk old), JE101 (4 wk old), and JG-42 (4 wk old). (B) Genomic DNA from the thymi of mice from lines A, JA, JE, and JG were amplified by PCR using primers 5 and 6 (top), primers 1 and 4 (middle) and primers 2 and 4 (bottom). The mice analyzed were A-866, JA-81, JE-101, and JG-42 (all 4 wk old).

PCR analysis of E_δ and δE3,4 minilocus rearrangement. Genomic DNA from thymi of wild-type E_δ mice from lines A, B, and C, and from δE3,4 mice from lines JA, JE, and JG were amplified by PCR using primers 5 and 6 (top), primers 1 and 3 (middle) and primers 2 and 3 (bottom). Southern blots were developed using radiolabeled C_δ, V_δ1, or V_δ2 cDNA probes. The mice analyzed were A-765 (4 wk old), B-31 (8 wk old), C-114 (4 wk old), JA-81 (4 wk old), JE101 (4 wk old), and JG-42 (4 wk old). (B) Genomic DNA from the thymi of mice from lines A, JA, JE, and JG were amplified by PCR using primers 5 and 6 (top), primers 1 and 4 (middle) and primers 2 and 4 (bottom). The mice analyzed were A-866, JA-81, JE-101, and JG-42 (all 4 wk old).