Figure 3.

mAb L11 inhibits T cell binding to HEV in vivo. (A) L11 pretreatment inhibits T (closed bars) but not B (open bars) cell localization to PLN, MLN, PP, and spleen 1 h after intravenous injection. TRITClabeled lymphocytes were treated with L11 or isotype-matched negative control mAb for 20 min, washed, and 5 × 10⁷ cells injected via the tail vein. After 1 h, peripheral blood was collected by heart puncture and lymphocyte suspensions prepared from lymphoid organs were stained with FITC-conjugated anti–Thy-1, spiked with internal standard fluorescent beads for enumeration, and analyzed by two-color flow cytometry. (B) Homing of L11-pretreated T cells returns to that of control cells over 48 h. Redistribution studies were performed as described for A using cells labeled with 10 mM Cell-Tracker Orange.