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. 1997 Apr 21;185(8):1481–1486. doi: 10.1084/jem.185.8.1481

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ATP-depletion and the shape of cell death: apoptosis or necrosis. Cells, in a glucose-free medium supplemented with 2 mM pyruvate, were incubated with solvent (control) (squares), 1.2 μM STS (triangles), or 100 ng/ml αCD95 (circles). Pretreatment with 2.5 μM oligomycin (olig) is shown by the open symbols; (a) intracellular ATP concentration was measured at the time indicated and expressed as percentage of untreated controls (1.6 nmol/mg protein; 4.5 nmol/10⁶ cells). ATP concentrations in cells treated with oligomycin plus STS or αCD95 did not differ from those of cells treated with oligomycin alone (open squares); (b and c) the percentage of apoptotic and necrotic cells was determined after staining of cultures with H-33342 plus SYTOX; in d, Jurkat cell were incubated with 1.2 μM STS (left), STS plus oligomycin (middle), or STS plus oligomycin plus 5 mM glucose (right) for 4 h, before staining with SYTO-13 (green) and EH-1 (red). Data are means ± SD of triplicate determinations and representative of at least four experiments.

ATP-depletion and the shape of cell death: apoptosis or necrosis. Cells, in a glucose-free medium supplemented with 2 mM pyruvate, were incubated with solvent (control) (squares), 1.2 μM STS (triangles), or 100 ng/ml αCD95 (circles). Pretreatment with 2.5 μM oligomycin (olig) is shown by the open symbols; (a) intracellular ATP concentration was measured at the time indicated and expressed as percentage of untreated controls (1.6 nmol/mg protein; 4.5 nmol/10⁶ cells). ATP concentrations in cells treated with oligomycin plus STS or αCD95 did not differ from those of cells treated with oligomycin alone (open squares); (b and c) the percentage of apoptotic and necrotic cells was determined after staining of cultures with H-33342 plus SYTOX; in d, Jurkat cell were incubated with 1.2 μM STS (left), STS plus oligomycin (middle), or STS plus oligomycin plus 5 mM glucose (right) for 4 h, before staining with SYTO-13 (green) and EH-1 (red). Data are means ± SD of triplicate determinations and representative of at least four experiments.