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. 1997 May 5;185(9):1661–1670. doi: 10.1084/jem.185.9.1661

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Expression of Src-family kinases in hck ^−/− fgr ^−/− lyn ^−/− macrophages. Total protein extracts (30 μg) obtained from BMDMs of wildtype (W) or triple mutant (T) mice were electrophoresed on 10% SDSPAGE and transferred to nitrocellulose as described in Materials and Methods. Membranes were blotted with different antibodies against Srcfamily kinases. Brain (B) and lymphocyte (L) extracts used as positive controls for antibody binding. Similar to these results, expression of other Src-family kinases was not observed in triple mutant PEMs (data not shown).