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. 1997 May 5;185(9):1661–1670. doi: 10.1084/jem.185.9.1661

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Secretion of IL-1, IL-6, and TNF-α in response to LPS and IFN-γ stimulation. PEMs or BMDMs from either wild-type or triple mutant mice were cultured in 96-well dishes (5 × 10⁵ cells/well) and stimulated with LPS and IFN-γ (20 ng/ml) for 12 h. The concentration of IL-1, IL-6, and TNF-α in the culture media was determined by ELISA. Determinations were performed on triplicate samples and plotted ± SD. Results shown are averaged data from three independent experiments. WT + IFN-γ (squares); hck ^−/− fgr ^−/− lyn ^−/−+ IFN-γ (diamonds).