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. 1997 May 5;185(9):1661–1670. doi: 10.1084/jem.185.9.1661

Figure 6.

Figure 6

Protein-tyrosine phosphorylation after LPS/IFN-γ stimulation. PEMs and BMDMs from wild-type and hck / fgr / lyn / mice were stimulated with LPS (10 ng/ml) and IFN-γ (20 ng/ml) for the different time points indicated. Total cell lysates were prepared and separated on 10% SDS-PAGE and transferred to nitrocellulose as described in Materials and Methods. Filters were blotted with antiphosphotyrosine with 4G10, followed by peroxidase-conjugated secondary antibody and chemiluminescent detection.