Table 1.

Comparison of the Phenotype of Lymph Node CD4⁺ Cells from PBS- and TNF-treated Transgenic Mice

In vivo treatment		Whole lymph node CD4				Cell surface marker
						CD4 gated population
						Vβ8				CD69				CD44				CD25				CD45RB
		MFL		%		MFL		%		MFL		%		MFL		%		MFL		%		RBhi		RBlo
PBS		147.9		48.5		103.9		91.1		52.2		12.5		76.4		98.6		168.5		18.0		80.3		19.7
TNF		157.4		47.7		108.4		90.6		53.4		15.7		95.7		99.3		229.8		19.8		80.9		19.1

BALB/c HNT-TCR transgenic mice were treated as described in Fig. 1. After 3 wk of injections, lymph nodes were harvested and cell suspensions stained with mAb specific for the indicated cell surface marker, before analysis by flow cytometry. Data are expressed both as mean log₁₀ fluorescence (MFL) and % CD4⁺ cells staining positive, as determined by two-color immunofluorescence. MFL and % data for CD4 expression are shown for whole lymph node populations. Data are representative of three independant determinations.