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The binding of purified CEA to pEXI. Equal numbers of pGEM (Opa−) and pEXI (OpaI) were suspended in RPMI containing equal amounts of CEA. After incubation, the bacteria were recovered by centrifugation, washed, and lysed, and the lysates were subjected to SDS-PAGE and transfered to Immobilon-P membrane. The CEA bound on the bacteria was detected by anti-CD66 mAb (COL-1). Much more CEA was bound by pEXI than by pGEM.
