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. 1997 May 5;185(9):1557–1564. doi: 10.1084/jem.185.9.1557

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Multiple Opa proteins promote adherence to HeLa-CEA. pGEM, pEXA, pEXB, pEXC, pEXH, and pEXI were incubated with HeLaCEA (A) for 4 h. All of Opa⁺ E. coli adhered to the cell lines. (B) Addition of COL-1 antibody (CD66) inhibited adherence of OpaI and OpaC E. coli to HeLaCEA. Control antibody IB4 (CD18) had no effect when added at the same final concentration (25 μg/ml). The interaction of pEXI and pEXC with HeLa-CEA was for 2 h and only accounted for the lower bacterial counts.

Multiple Opa proteins promote adherence to HeLa-CEA. pGEM, pEXA, pEXB, pEXC, pEXH, and pEXI were incubated with HeLaCEA (A) for 4 h. All of Opa⁺ E. coli adhered to the cell lines. (B) Addition of COL-1 antibody (CD66) inhibited adherence of OpaI and OpaC E. coli to HeLaCEA. Control antibody IB4 (CD18) had no effect when added at the same final concentration (25 μg/ml). The interaction of pEXI and pEXC with HeLa-CEA was for 2 h and only accounted for the lower bacterial counts.