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Cytosine-mediated opening of M1G to N2-(3-oxo-1-propenyl)-dG is reflected by changes in scalar coupling constants. (A) 1H spectrum of N2-(3-oxo-1-propenyl)-dG resonances H6, H7, and H8 at pH 10. (B) 1H spectrum of M1G resonances H6, H7, and H8 at pH 7. (C) Expanded region of a 1H double quantum-filtered correlated spectroscopy spectrum of N2-(3-oxo-1-propenyl)-dG crosspeaks at pH 7.
