Table 2.

Effects of Immune Cell Transfer in LCMV-carrier Mice

Treatment*		n		CD8 Cell No. × 106		Mean FSC		CD44 MFI‡		MEL-14 MFI
		Experiment No. 1 (2 × 107 cells transfered)
Host (naive transfer)		1		15		642		ND		ND
Host (immune transfer)		3		12 ± 3		639 ± 3		ND		ND
Donor (immune transfer)		3		5.3 ± 2		717 ± 11		ND		ND
		Experiment No. 2 (4 × 107 cells transferred§)
Host (naive transfer)		2		12 ± 2		563 ± 5		760 ± 24		449 ± 19
Host (immune transfer)		3		6.1 ± 1		578 ± 8		723 ± 85		433 ± 12
Donor (immune transfer)		3		4.5 ± 1		726 ± 4		846 ± 54‖		318 ± 5
Host + poly I:C (naive transfer)		2		10 ± 1		578 ± 1		749 ± 2		435 ± 7
Host + poly I:C (immune transfer)		3		4.0 ± 0.6		565 ± 18		783 ± 74		423 ± 12
Donor + poly I:C (immune transfer)		3		4.0 ± 0.6		720 ± 23		885 ± 6		316 ± 7
		Experiment No. 3 (3 × 107 cells transferred§)
Host (naive transfer)		2		7.8 ± 1		498 ± 13		533 ± 18		479 ± 7
Host (immune transfer)		4		4.9 ± 0.7		494 ± 7		512 ± 11		505 ± 30
Donor (immune transfer)		4		3.7 ± 0.8		630 ± 7		698 ± 15		385 ± 21
Host + poly I:C (naive transfer)		2		7.0 ± 2		496 ± 16		505 ± 16		485 ± 6
Host + poly I:C (immune transfer)		3		3.8 ± 0.6		471 ± 11		447 ± 22¶		457 ± 19
Donor + poly I:C (immune transfer)		3		3.8 ± 0.7		627 ± 21		666 ± 22		350 ± 27

^*LCMV-carrier mice received the indicated number of either LCMV-immune (immune transfer) or naive spleen cells (naive transfer) as detailed in Materials and Methods. They were then either left untreated or treated with 100 μg poly I:C intraperitoneally after transfer. 6 d after adoptive transfer, the spleens were harvested and stained as stated in the text.  

^‡MFI, mean fluorescent intensity.  

^§The differences between the mean FCS and MEL-14 MFI of host (naive transfer) and donor cells are all significant at P <0.005 for FSC and P <0.009 for MEL-14, as determined by Student's t test.  

^‖Different (P <0.13) from host (naive transfer).  

^¶Different from both host (naive transfer) (P <0.02) and host + poly I:C (naive transfer) (P <0.05).