Figure 7.

TCR determinant(s) within B5 are naturally processed and presented. DBA/1LacJ mice were immunized with 7 nmol of B5 in CFA. 10 d later, draining lymph node cells were assayed for proliferation in response to varying concentration of different antigens (3.5–14 μM). [³H]thymidine incorporation, measured by standard techniques, at an optimum concentration, 7 μM, is shown. Responses in lymph node cells pooled from two mice are shown. B5 and B3 are synthetic TCR peptides, whereas Vβ8.2–Vα4 and Vα4 are scTCR molecules containing both V domains or only a single V domain, respectively.